PKM2 Mouse Polyclonal Antibody

• Catalog Number: orb101937
• Category: Antibodies
• Description: PKM2 Mouse Polyclonal Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PKM2 (51-150/531aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: PKM
• UniProt ID: P14618
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (orb101937) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Hela Cell (Human) Lysate at 40 ug, NIH/3T3 Cell (Mouse) Lysate at 40 ug, Muscle (Mouse) Lysate at 40 ug, Jurkat Cell (Human) Lysate at 40 ug, Primary: Anti-PKM2 (orb101937) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: U87MG (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-PKM2 (orb101937) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-PKM2 (orb101937) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Tissue/cell: Human nasopharyngeal carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (orb101937) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U-87MG cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (orb101937) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.