PKM2 Antibody (C-term L398)

• Catalog Number: orb1929507
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18405
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937 Da
• Target: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the C-terminal region of human PKM2.
• UniProt ID: P14618
• NCBI: NP_001193728.1, NP_001193726.1, NP_872271.1, NP_001193725.1, NP_001193727.1, NP_002645.3, NP_872270.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PKM2 Antibody (C-term L398) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PKM2 Antibody (C-term L398) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 Antibody (C-term L398) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of PKM2 Antibody (C-term L398) in K562 cell line lysates (35 ug/lane). PKM2 (arrow) was detected using the purified Pab.

Anti-PKM2 Antibody (C-term L398) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.