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Catalog Number | orb1929508 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB15339 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rabbit |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 57937 Da |
Target | This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-489 amino acids from the C-terminal region of human PKM2. |
UniProt ID | P14618 |
NCBI | NP_001193728.1, NP_001193726.1, NP_872271.1, NP_001193725.1, NP_001193727.1, NP_002645.3, NP_872270.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Pyruvate kinase PKM, Cytosolic thyroid hormone-bin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PKM2 antibody (C-term C474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-PKM2 Antibody (C-term C474) in CEM cell line lysates (35 ug/lane). PKM2 (arrow) was detected using the purified Pab.