PKM1 Antibody (C-term L398)

• Catalog Number: orb1929222
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18404
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57937 Da
• Target: This PKM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the C-terminal region of human PKM1.
• UniProt ID: P14618
• NCBI: NP_872271.1, NP_001193727.1, NP_872270.1, NP_001193726.1, NP_001193725.1, NP_001193728.1, NP_002645.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35 ug/lane). PKM1 (arrow) was detected using the purified Pab.

Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35 ug/lane). PKM1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.