PKC zeta (phospho-T560) antibody

• Catalog Number: orb214448
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKCZ
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T560 of human PKC zeta protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKCZ
• Entrez: 5590, 25522, 18762
• UniProt ID: Q05513, Q02956, P09217
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PKC2 antibody, anti Protein kinase C zeta typ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKC zeta (Phospho-T560) expression in HEK293T (A), Hela (B), H446 (C), mouse kidney (D), mouse testis (E), rat kidney (F), rat testis (G) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 78 kD)

Immunohistochemical analysis of PKC zeta (Phospho-T560) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.