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Catalog Number | orb556739 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PKC alpha |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PKC alpha. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Concentration | 0.69 mg/ml |
Dilution range | WB: 1:500-1:3000 , IHC-P: 1:100-1:1000 |
Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Purity | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
MW | 77 |
Target | PKC alpha |
UniProt ID | P17252 |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
Buffer/Preservatives | 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol |
Alternative names | protein kinase C alpha , AAG6 , PKC-alpha , PKCA , Read more... |
Note | For research use only |
Application notes | Positive control: H1299 , Jurkat , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Rat brain using PKC alpha antibody
Western blot analysis of Various whole cell using PKC alpha antibody
Western blot analysis of Various whole cell using PKC alpha antibody
Western blot analysis of Various whole cell using PKC alpha antibody
Western blot analysis of Whole cell extract using PKC alpha antibody
Western blot analysis of H1299 cells using PKC alpha antibody
Western blot analysis of Molt-4 cells using SHC4 antibody
IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Equine, Gallus, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |