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Catalog Number | orb1255367 |
---|---|
Category | Antibodies |
Description | PIWIL4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant protein of human PIWIL4 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 110kDa |
Target | PIWIL4 |
UniProt ID | Q7Z3Z4 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | HIWI2, MIWI2, piwi-like protein 4, piwi-like 4, te Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PIWIL4 antibody (orb1255367) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using PIWIL4 antibody (orb1255367) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PIWIL4 antibody (orb1255367) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PIWIL4 antibody (orb1255367). Blue: DAPI for nuclear staining.
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