PIWIL1 antibody

• Catalog Number: orb340827
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PIWIL1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human HIWI1
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:20-1:100, IP: 1:20-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PIWIL1
• Entrez: 9271, 57749
• UniProt ID: Q96J94, Q9JMB7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HIWI; Piwi-like protein 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIWI1 expression in HepG2 (A), SKOV3 (B), mouse testis (C), rat testis (D) whole cell lysates. (Predicted band size: 95; 98 kD; Observed band size: 102; 110 kD)

Immunofluorescent analysis of HIWI1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.