PITRM1 antibody

• Catalog Number: orb381958
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PITRM1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human PITRM1
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PITRM1
• Entrez: 69617, 10531
• UniProt ID: Q5JRX3, Q8K411
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KIAA1104; MP1; Presequence protease mitochondrial;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PITRM1 expression in A549 (A), MCF7 (B), mouse liver (C), mouse kidney (D), rat heart (E) whole cell lysates. (Predicted band size: 106; 117 kD; Observed band size: 117 kD)

Immunofluorescent analysis of PITRM1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).