PIK3R3 Antibody

• Catalog Number: orb1258728
• Category: Antibodies
• Description: PIK3R3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein of human PIK3R3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: PIK3R3
• UniProt ID: Q92569
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (orb1258728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (orb1258728) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (orb1258728) at dilution of 1:100. Blue: DAPI for nuclear staining.