PICK1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb612210
• Category: Antibodies
• Description: PICK1 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 4G1
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PICK1
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: PICK1
• UniProt ID: Q9NRD5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PICK 1; Pick1; PICK1_HUMAN; PRKCA binding protein;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-PICK1 antibody, dilution: 1:100 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: SK-Br-3 cell lysate, Lane 2: 293 cell lysate, Primary: Anti-PICK1 (orb612210) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 46 kD, Observed band size: 52 kD.