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Catalog Number | orb152169 |
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Category | Antibodies |
Description | Rabbit polyclonal to Picollo (FITC). Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1.. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant Full length Protein, BC001304.1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000), IHC (1:400) |
Conjugation | FITC |
MW | 550kDa |
Target | Piccolo |
Entrez | 27445 |
UniProt ID | Q9Y6V0 |
NCBI | NP_055325.2 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS |
Alternative names | ACZ antibody, Aczonin antibody, Brain-derived HLMN Read more... |
Note | For research use only |
Application notes | 1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.
Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:1000.