PIAS1 Rabbit Polyclonal Antibody

• Catalog Number: orb577019
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PIAS1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PIAS1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 72kDa
• Target: PIAS1
• UniProt ID: O75925
• Protein Sequence: Synthetic peptide located within the following region: LPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISL
• NCBI: NP_057250
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: GBP, ZMIZ3, DDXBP1, GU/RH-II
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Lanes: Lane 1: Untreated human U251 lysate, Lane 2: IL-1beta treated human U251 lysate, Primary Antibody Dilution: 1:500, Secondary Antibody: Anti-rabbit-HRP, Secondary Antibody Dilution: 1:500, Gene Name: PIAS1.

WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: COLO205 cell lysate. There is BioGPS gene expression data showing that PIAS1 is expressed in COLO205.