PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559167
• Category: Antibodies
• Description: PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 34F12
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human PI3-kinase p110 subunit beta protein, around C-terminal 150aa
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:200, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 123 kDa
• Target: PIK3CB
• UniProt ID: Q24JU2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PI3-kinase p110 subunit beta; p110 BETA; p110Beta;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4% Paraformaldehyde-fixed MCF-7 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (PI3-kinase p110 subunit beta) monoclonal Antibody, unconjugated (orb559167) 1:200, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Endometrium Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated (orb559167) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-PI3-kinase p110 subunit beta (orb559167) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 123 kD, Observed band size: 110 kD.