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Catalog Number | orb12386 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to PI15. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:1000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 29.1 |
Target | PI15 (aa128-141) |
Entrez | 51050 |
Protein Sequence | RTGRYRSILQLVKP |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PI15 antibody Read more... |
Note | For research use only |
Application notes | WB: Approx 33kDa band observed in lysates of cell line HeLa (calculated MW of 29.1kDa according to NP_056970.1). Recommended concentration: 0.3-1µg/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.. IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml. |
Expiration Date | 12 months from date of receipt. |
orb12386 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb12386 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, IHC, WB | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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