Phospho-TAK1 (Thr187) Rabbit Polyclonal Antibody

• Catalog Number: orb7049
• Category: Antibodies
• Description: Phospho-TAK1 (Thr187) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TAK1 around the phosphorylation site of Thr187 HM(p-T)NN
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 67 kDa
• Target: MAP3K7
• UniProt ID: O43318
• RRID: AB_10922511
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TAK1 (phospho T187); TAK1(Phospho Thr187); TAK1(Ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-Phospho-TAK1 (Thr187) antibody (orb7049), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 20 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: 293T (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-TAK1 (Thr187) (orb7049) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 75 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-TAK1 (Thr187) (orb7049) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67 kD, Observed band size: 70 kD.