Phospho-TAK1 (Thr184 + Thr187) Rabbit Polyclonal Antibody

• Catalog Number: orb7050
• Category: Antibodies
• Description: Phospho-TAK1 (Thr184 + Thr187) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TAK1 around the phosphorylation site of Thr184/187 IQ(p-T)HM(p-T)NN
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:50-1000, IHC-F=1:50-1000, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 67 kDa
• Target: MAP3K7
• UniProt ID: O43318
• RRID: AB_10932075
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TAK1(Phospho T184 + T187); TAK1(Phospho Thr184 + T
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zheng, Xichuan et al. TAK1 accelerates transplant arteriosclerosis in rat aortic allografts by inducing autophagy in vascular smooth muscle cells Atherosclerosis, 343, 19/10/2023 (2022)

PubMed: 35078016

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-TAK1 (Thr184/187) Polyclonal Antibody, Unconjugated (orb7050) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-TAK1 (Thr184/187) Polyclonal Antibody, Unconjugated (orb7050) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.