Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody

• Catalog Number: orb608174
• Category: Antibodies
• Description: Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705 AP(p-Y)LK
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 85 kDa
• Target: STAT3
• UniProt ID: P40763
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: STAT3 (phospho Y705); p-STAT3 (phospho Y705); Phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (orb608174) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (orb608174) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Jurkat treated with IFN-α (100 ng/ml, 5 min), Primary: Anti-phospho-STAT3 (Tyr705) (orb608174) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kDa, Observed band size: 80 kDa.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-phospho-STAT3 (Tyr705) (orb608174) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 80 kD.