Phospho-Stat3 (Tyr705) (B12) rabbit mAb Antibody

• Catalog Number: orb1946365
• Category: Antibodies
• Description: Phospho-Stat3 (Tyr705) (B12) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: Stat3Y705-B12
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human phospho Stat3
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P40763
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Signal transducer and activator of transcription 3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with IFNα IL-4 and pervanadate (green) treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. #orb1946365.

Stat3Y705-B12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (green) Cat. #orb1946365.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #orb1946365.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Stat3 (Y705) antibody Stat3Y705-B12 (Cat. #orb1946365) or Company C antibody at 0.62 μg/mL (manufacturer's recommended concentration).