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Catalog Number | orb548648 |
---|---|
Category | Antibodies |
Description | Phospho-PAK1/2/3 (S144+S141+S139) PAK3 Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | CFF-16 |
Tested applications | FC, ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human Phospho-PAK1/2/3 (S144+S141+S139) |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 62310 MW |
UniProt ID | Q13177, O75914, Q13153 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Serine/threonine-protein kinase PAK 3;2.7.11.1;Bet Read more... |
Note | For research use only |
Application notes | WB 1:1000-1:10000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50 |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded mouse brain, using Phospho-PAK1/2/3 (S144+S141+S139) Antibody (orb548648) PAK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAK3 Antibody (orb548648) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
Western blot analysis of Phospho-PAK1/2/3 expression in HeLa Cell lysate treated with lambda phosphatase (orb548648). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 monoclonal antibody (Catalog # orb548648) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for PAK3.
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