Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody

• Catalog Number: orb6544
• Category: Antibodies
• Description: Phospho-Nrf2 (Ser40) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 DF(p-S)QR
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: NFE2L2
• UniProt ID: Q16236
• RRID: AB_10928503
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Helia Aghamiri # 1 2, Afrooz Mohammadgholi-Beiki # 3, Rojin Rashidian 1 3, Manijeh Motevalian 1 3, Parvaneh Rahimi-Moghaddam 3, Mohammad Sheibani # 4 5, Majid Jafari-Sabet Zhumeria majdae essential oil attenuates TNBS-induced colitis in rats by regulating inflammatory and apoptotic pathways Inflammopharmacology, (2024)

PubMed: 39312096

Applications

WB

Reactivity

Rat

Gupta, Mahesh et al. Dwindling of cardio damaging effect of isoproterenol by Punica granatum L. peel extract involve activation of nitric oxide-mediated Nrf2/ARE signaling pathway and apoptosis inhibition Nitric Oxide, 50, 105-113 (2015)

PubMed: 26363155

Applications

IHC

Reactivity

Rat

Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (orb6544), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (orb6544) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (orb6544) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (orb6544) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Lane 5: B16 (Human) Cell Lysate at 30 ug, Lane 6: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Nrf2 (Ser40) (orb6544) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 135 kD.