Phospho-NPM (Ser4) (A1) rabbit mAb Antibody

• Catalog Number: orb1945998
• Category: Antibodies
• Description: Phospho-NPM (Ser4) (A1) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: NPMS4-A1
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser4 of human phospho-NPM
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P06748
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Nucleophosmin, Nucleolar phosphoprotein B23, numar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of C6 cells untreated (red) or treated with nocodazole (green) using Phospho-NPM (Ser4) (A1) rabbit mAb NPMS4-A1 #orb1945998 at 0.01 ug/mL, or concentration-matched rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with nocodazole (blue).

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or treated with imatinib and blocked with phospho-peptide (black) or treated with pervanadate and blocked with phospho-peptide (gold) or treated with imatinib and blocked by non-phospho-peptide (dark blue) or treated with pervanadate and block with non-phospho-peptide (purple) using Phospho-NPM (Ser4) antibody NPMS4-A1 at 0.01 ug/mL, Cat#orb1945998.

Western blot analysis of extract from HeLa cells untreated or treated with nocodazole using Phospho-NPM (Ser4) antibody NPMS4-A1 at 01 ug/mL, Cat# orb1945998