Phospho-MEK1 + MEK2 (Ser222) Rabbit Polyclonal Antibody

• Catalog Number: orb5172
• Category: Antibodies
• Description: Phospho-MEK1 + MEK2 (Ser222) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MEK1/2 around the phosphorylation site of Ser222 AN(p-S)FV
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-2000, IHC-P=1:100-500, ELISA=1:5000-10000, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: MAP2K2
• UniProt ID: P36507
• RRID: AB_10953411
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MEK1(phospho-Ser222); p-MEK-1/2(phospho-Ser222); p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MEK1 + MEK2 (Ser222)) Polyclonal Antibody, Unconjugated (orb5172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MEK1 + MEK2 (Ser222)) Polyclonal Antibody, Unconjugated (orb5172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MEK1 + MEK2 (Ser222)) Polyclonal Antibody, Unconjugated (orb5172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Phospho-MEK1 + MEK2 (Ser222 (orb5172) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD.