Phospho-IL-1R1 (Tyr496) Rabbit Polyclonal Antibody

• Catalog Number: orb6228
• Category: Antibodies
• Description: Phospho-IL-1R1 (Tyr496) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Canine, Equine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496 QD(p-Y)EK
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 63 kDa
• Target: IL1R1
• UniProt ID: P14778
• RRID: AB_10926486
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IL1 Receptor I (phospho Tyr496); Antigen CD121a; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-phospho-IL-1R1 (Tyr496) antibody (orb6228), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Intestine (Mouse) Lysate at 40 ug, Primary: Anti-phospho-IL-1R1 (Tyr496) (orb6228) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD.