Phospho-HSP27 (Ser82) (CB2) rabbit mAb Antibody

• Catalog Number: orb1946056
• Category: Antibodies
• Description: Phospho-HSP27 (Ser82) (CB2) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: HSP27S82-CB2
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human phospho Hsp27
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P04792
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Heat shock protein beta-1, HspB1, 28 kDa heat shoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell extract, untreated or treated with UV plus TPA using Phospho-HSP27 (Ser82) antibody HSP27S82-CB2 at 5 ng/mL. Cat. # orb1946056

Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) using Phospho-HSP27-CB2 (Ser82) antibody HSP27S82-CB2 at 1.0 ng/mL. Cat. #orb1946056.

Peptide blocking flow cytometric analysis of HeLa cells unstained and untreated as negative control (light blue) or stained and untreated (red) or treated with UV + plus TPA (green) or untreated and blocked with phospho-peptide (black) or UV plus TPA blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV plus TPA and blocked with non-phospho peptide (purple) using Phospho-HSP27 (Ser82) antibody HSP27S82-CB2 at 10 μg/mL, Cat#orb1946056.