Phospho-HSP27 (Ser78) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559187
• Category: Antibodies
• Description: Phospho-HSP27 (Ser78) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 5E1
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78 AL(p-S)RQ
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-200, ICC/IF=1:50, IF=1:50-200, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: HSPB1
• UniProt ID: P04792
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Hsp27(S78); Hsp27 (phospho S78); p-Hsp27 (phospho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa + Anisomycin (-), 2 HeLa +, Anisomycin (+), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 23 kDa, Observed MW: 27 kDa.

Cell line: A: HeLa, B HeLa + Anisomycin (+), Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% Triton X-100, Primary Ab, dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb559187.

Tissue: Human breast cancer, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab, dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559187.

Tissue: Human cervical cancer, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab, dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559187.

Tissue: Human tonsil, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab, dilution: 1:500, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559187.