phospho Histone H2A.X (Ser139), monoclonal antibody

• Catalog Number: orb1973161
• Category: Antibodies
• Description: phospho Histone H2A.X (Ser139), monoclonal antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human
• Dilution range: Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:200 Immunohistochemistry (Paraffin) 1:50 – 1:100
• Conjugation: Unconjugated
• Target: phospho Histone H2A.X (Ser139), monoclonal antibody
• Storage: Long time storage is recommended at -20°C Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

phospho Histone H2A.X (Ser139), monoclonal antibody at 1/500 dilution. Lysates/at 35 μg per lane. NC blot was then blocked for an hour before being incubated with orb1973161 overnight at 4°C.

Immunofluorescence of cell. phospho Histone H2A.X (Ser139), monoclonal antibody (orb1973161): 1-200.

Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue.