Phospho-GluR1 (Ser849) Rabbit Polyclonal Antibody

• Catalog Number: orb1844
• Category: Antibodies
• Description: Phospho-GluR1 (Ser849) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser849 QQ(p-S)IN
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 98 kDa
• Target: GRIA1
• UniProt ID: P42261
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GluR1(phospho Ser849); GluR1(phospho S849); p-GluR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GluR1 (Ser849)) Polyclonal Antibody, Unconjugated (orb1844) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, U251 (Human) Lysate at 30 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 130 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 128 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, U251 (Human) Lysate at 30 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 128 kD.