Phospho-FXYD1/PLM (Ser83) Antibody

• Catalog Number: orb1095345
• Category: Antibodies
• Description: Phospho-FXYD1/PLM (Ser83) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human FXYD1/PLM around the phosphorylation site of Ser83
• Concentration: ~1mg/ml
• Dilution range: WB 1:500-1:2000, IHC 1:50-1:200
• Purity: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
• Conjugation: Unconjugated
• Storage: Store at -20 °C. Stable for 12 months from date of receipt
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Note: For research use only
• Application notes: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight
• Expiration Date: 12 months from date of receipt.

orb1095345 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab