Phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Rabbit Polyclonal Antibody

• Catalog Number: orb783430
• Category: Antibodies
• Description: Rabbit Anti-phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse ERK1/2 around the phosphorylation site of Thr202/Tyr204 FL(p-T)E(p-Y)VA
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test,ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: MAPK3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Erk1 (pT202/pY204) + Erk2 (pT185/pY187); p44/42 MA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) antibody (orb783430), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

cells: human.

Publition Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes, IF: 2.5.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-ERK 1+ ERK 2) Polyclonal Antibody, Unconjugated (orb783430) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 (Thr202Tyr204) + ERK2 (Thr183Tyr185)) Polyclonal Antibody, Unconjugated (orb783430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 (Thr202Tyr204) + ERK2 (Thr183Tyr185)) Polyclonal Antibody, Unconjugated (orb783430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 (Thr202Tyr204) + ERK2 (Thr183Tyr185)) Polyclonal Antibody, Unconjugated (orb783430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-ERK 1+ ERK 2) Polyclonal Antibody, Unconjugated (orb783430) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 (Thr202Tyr204) + ERK2 (Thr183Tyr185)) Polyclonal Antibody, Unconjugated (orb783430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-ERK1 (Thr202/Tyr204)+ERK2 (Thr183/Tyr185) Polyclonal Antibody, Unconjugated (orb783430) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.