Phospho-CHEK2 (Thr68) Rabbit Polyclonal Antibody

• Catalog Number: orb5862
• Category: Antibodies
• Description: Phospho-CHEK2 (Thr68) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68 VS(p-T)QE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CHEK2
• UniProt ID: O96017
• RRID: AB_10920737
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CHK2_HUMAN; Serine/threonine-protein kinase Chk2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-CHK2 (Thr68)) Polyclonal Antibody, Unconjugated (orb5862) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Colon carcinoma (Human) Lysate at 30 ug, Primary: Anti-Phospho-CHK2 (Thr68) (orb5862) at 1:200dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 61kD, Observed band size: 61kD.

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CHK2 (Thr68) Polyclonal Antibody, Unconjugated (orb5862) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.