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Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Catalog Number: orb6571

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6571
CategoryAntibodies
DescriptionPhospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human P21 around the phosphorylation site of Ser130 EG(p-S)PG
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW18 kDa
TargetCDKN1A
UniProt IDP38936
RRIDAB_10918248
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCDKN1A/p21 (phospho Ser130); CDKN1A/p21 (phospho S
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Blank control: 293T. Primary Antibody (green line): Rabbit Anti-phospho-CDKN1A/p21 (Ser130) antibody (orb6571), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1A (Ser130)) Polyclonal Antibody, Unconjugated (orb6571) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-CDKN1A (Ser130)) Polyclonal Antibody, Unconjugated (orb6571) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1A (Ser130)) Polyclonal Antibody, Unconjugated (orb6571) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1A (Ser130)) Polyclonal Antibody, Unconjugated (orb6571) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1A) Polyclonal Antibody, Unconjugated (orb6571) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CDKN1A (Ser130) Polyclonal Antibody, Unconjugated (orb6571) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-CDKN1A/p21 (Ser130) Rabbit Polyclonal Antibody

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CDKN1A (Ser130) Polyclonal Antibody, Unconjugated (orb6571) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.