Phospho-c-Cbl (Tyr774) (R4C5) rabbit mAb Antibody

• Catalog Number: orb1946069
• Category: Antibodies
• Description: Phospho-c-Cbl (Tyr774) (R4C5) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: CblY774-R4C5
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human phospho c-Cbl
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P22681
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: E3 ubiquitin-protein ligase CBL, Casitas B-lineage
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-c-Cbl (Tyr774) antibody CblY774-R4C5 at 0.01 μg/mL. Cat. #orb1946069.

Western blot analysis of extracts from Ramos or Jurkat cells, imatinib or pervanadate treated using Phospho-c-Cbl (Tyr774) antibody c-CblY774-R4C5 at 0.1 ug/mL, Cat# orb1946069

Western blot analysis of Jurkat cell extract treated with imatinib or with pervanadate using 0.1 μg/mL Phospho-c-Cbl (Tyr774) antibody CblY774-R4C5 Cat. #orb1946069 or Company A antibody at 0.04 μg/mL (manufacturer's recommended concentration) developed using the same exposure.