Phospho-Btk (Tyr223) (B4) rabbit mAb Antibody

• Catalog Number: orb1946336
• Category: Antibodies
• Description: Phospho-Btk (Tyr223) (B4) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: BtkY223-B4
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q06187
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Bruton tyrosine kinase, Tyrosine-protein kinase BT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa IL4 and PV (green) using 5 ng/mL of Phospho-Btk (Tyr223) antibody BtkY223-B4 Cat. #orb1946336.

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or IFNα + IL4 + pervanadate-treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.025μg/mL. Cat. #orb1946336.

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with IFNα + IL4 + pervanadate (orange) using 0.05 μg/mL isotype control Cat. #orb1946241, or untreated (red) or treated (green) using Phospho-Btk (Tyr223) antibody BtkY223-B4 at 0.05μg/mL. Cat. #orb1946336.