Phospho-Bcl-xL (Thr47) Rabbit Polyclonal Antibody

• Catalog Number: orb155811
• Category: Antibodies
• Description: Phospho-Bcl-xL (Thr47) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Guinea pig, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human BCL2L1 around the phosphorylation site of Thr47 E(p-T)PS
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: BCL2L1
• UniProt ID: Q07817
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BCL2L1(phospho T47); BCL2L1(phospho Thr47); p-BCL2
• Background: _CLIENT_OVERRIDE_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Bcl-xL (Thr47)) polyclonal Antibody, Unconjugated (orb155811) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-BCL2L1) Polyclonal Antibody, Unconjugated (orb155811) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Bcl-xL (Thr47)) Polyclonal Antibody, Unconjugated (orb155811) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-p-Bcl-xL (Thr47) (orb155811) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26kD, Observed band size: 24kD.