Phospho-Bax (Ser184) Rabbit Polyclonal Antibody

• Catalog Number: orb4658
• Category: Antibodies
• Description: Phospho-Bax (Ser184) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Bax around the phosphorylation site of Ser184 TA(p-S)LT
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg /test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BAX
• UniProt ID: Q07812
• RRID: AB_10932623
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Bax (phospho S184); Bax (phospho Ser184); p-Bax (S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

de Vicente, Larissa G. et al. Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts Life Sci., 240, 117107 (2020)

PubMed: 31785241

Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-Bax (Ser184) antibody (orb4658), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Bax (Ser184)) Polyclonal Antibody, Unconjugated (orb4658) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

phosphopeptide, non phosphopeptide.

Sample: Brain (Mouse) lysate at 30 ug, Primary: Anti-phospho-Bax (Ser184) (orb4658) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 21kD, Observed band size: 21kD.

Sample: Testis (Mouse) Tissue Lysate at 30 ug, Primary: Anti-phospho-Bax (Ser184) (orb4658) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 22 kD.