Phospho-ATM (Ser1981) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb608078
• Category: Antibodies
• Description: Phospho-ATM (Ser1981) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 3F10
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ATM around the phosphorylation site of Ser1981 EG(p-S)Q
• Concentration: 1mg/ml
• Dilution range: WB=1:300-1000, IHC-P=1:50-100, IHC-F=1:50-100, ICC/IF=1:50-100, IF=1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 370 kDa
• Target: ATM
• UniProt ID: Q13315
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ATM(Phospho-Ser1981); ATM (phospho S1981); AT comp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: HepG2 cell lysates, Primary: Anti-phospho-ATM (Ser1981) (orb608078) at 1:1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:20000 dilution, Predicted band size: 370 kD, Observed band size: 350 kD.

Sample: Lane 1: Normal Human HeLa cell lysates, Lane 2: HeLa cell treated with 1 µM camptothecin (CPT) for 1 hour, Primary: Anti-phospho-ATM (Ser1981) (orb608078) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 370 kDa, Observed band size: 65 kDa.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.