Phospho-ATF2 (T71) Rabbit Monoclonal Antibody

• Catalog Number: orb548618
• Category: Antibodies
• Description: Phospho-ATF2 (T71) Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: FOO-1
• Tested applications: ICC, IF, IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Phospho-ATF2 (T71)
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54537 MW
• UniProt ID: P15336
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cyclic AMP-dependent transcription factor ATF-2;cA
• Note: For research use only
• Application notes: WB 1:500-1:2000ICC/IF 1:50-1:200IP 1:30
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

ICC staining of Phospho-ATF2 (T71) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS.