Phospho-AQP2 (Ser256) Rabbit Polyclonal Antibody

• Catalog Number: orb155711
• Category: Antibodies
• Description: Phospho-AQP2 (Ser256) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Monkey, Mouse, Porcine, Rabbit, Rat
• Reactivity: Canine, Human
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human AQP2 around the phosphorylation site of Ser256 RQ(p-S)VE
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: AQP2
• UniProt ID: P41181
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Aquaporin 2 (phospho S256); Aquaporin 2 (phospho S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Nooh, Mohammed M. et al. Involvement of PDZ-SAP97 Interactions in Regulating AQP2 Translocation in Response to Vasopressin in LLC-PK1 cells Am. J. Physiol. Renal Physiol., (2019)

PubMed: 31141395

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AQP2 (Ser256)) Polyclonal Antibody, Unconjugated (orb155711) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: MDCK (Dog) Cell Lysate at 30 ug, Primary: Anti-phospho-AQP2 (Ser256) (orb155711) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.