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Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (BF647)
Catalog Number: orb1587525
| Catalog Number | orb1587525 |
|---|---|
| Category | Antibodies |
| Description | Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody (BF647) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Porcine |
| Reactivity | Human, Mouse, Rat, Sheep |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC |
| Concentration | 1mg/ml |
| Dilution range | IF=1:100-500, Flow-Cyt=1ug/test |
| Conjugation | BF647 |
| Target | PRKAA2 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phosph Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blank control: Rat spleen. Primary Antibody (green line): Rabbit Anti-phospho-AMPK alpha-2 (Thr172) antibody (orb1587525), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
