Phospho-Akt(Ser473) antibody

• Catalog Number: orb395432
• Category: Antibodies
• Description: Mouse monoclonal antibody to Akt (Phospho-Tyr315).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5H4
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: v-akt murine thymoma viral oncogene homolog 1
• Dilution range: WB: 1:1000-1:4000; IHC: 1:50-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 60KD
• Target: Phospho-Akt(Ser473)
• UniProt ID: P31749
• Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.)
• Buffer/Preservatives: liquid
• Alternative names: AKT, AKT1, AKT1-phospho-S473, PKB, PKB ALPHA, PRKB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Jurkat cells were subjected to SDS PAGE followed by western blot with orb395432 (Phospho-Akt (S473) Antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb395432 (Phospho-Akt (S473) Antibody) at dilution of 1:200.