Phospho-AKT (Ser473)

• Catalog Number: orb1294322
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT (Phospho-ser473)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide corresponding to residues surrounding Ser473 of human AKT
• Dilution range: Western Blot 1:500- 1:1000 Immunofluorescence 1:200-1:400
• Conjugation: Unconjugated
• Target: Phospho-AKT (Ser473)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-AKT (pan) antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C.