PHO1 Antibody (N-term)

• Catalog Number: orb31634
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1749
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IHC-P: 1:50-100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 23012
• Target: APOBEC3A
• UniProt ID: P31941
• NCBI: NP_001180218.1, NP_663745.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-APOBEC3A antibody, anti-Phorbolin-1 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-hPHO1-M1 at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PHO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.