PHD2/EGLN1 Rabbit Antibody

• Catalog Number: orb1676193
• Category: Antibodies
• Description: PHD2/EGLN1 Rabbit Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2/EGLN1 (NP_071334.1).
• Dilution range: WB,1:500 - 1:2000|IHC-P,1:50 - 1:100|IF/ICC,1:50 - 1:200
• Conjugation: Unconjugated
• MW: 45kDa
• UniProt ID: Q9GZT9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMY
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of L929 cells using PHD2/EGLN1 Rabbit pAb (orb1676193) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using PHD2/EGLN1 Rabbit pAb (orb1676193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using PHD2/EGLN1 Rabbit pAb (orb1676193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PHD2/EGLN1 Rabbit pAb (orb1676193) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western blot analysis of various lysates using PHD2/EGLN1 Rabbit pAb (orb1676193) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.