PHD1 Antibody

• Catalog Number: orb1730082
• Category: Antibodies
• Description: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human PHD1/prolyl hydroxylase
• Dilution range: WB: 1/500-1/1000; IHC: 1/50-1/100; ICC: 1/50-1/200; RCM: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 44 kDa; Observed MW: 44 kDa
• Entrez: 112398
• UniProt ID: Q96KS0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: Estrogen-induced tag 6; HPH-3; PHD1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.