PHB2 Antibody

• Catalog Number: orb1263352
• Category: Antibodies
• Description: PHB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from human PHB2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: PHB2
• UniProt ID: Q99623
• NCBI: Q99623
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Prohibitin-2, B-cell receptor-associated protein B
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of PHB2-Y128in mouse NIH-3T3 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (Y128), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.