PHB Antibody

• Catalog Number: orb1264666
• Category: Antibodies
• Description: PHB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: PHB
• UniProt ID: P35232
• NCBI: P35232
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Prohibitin, PHB
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of PHB Antibody in Hela cell line lysates (35 ug/lane)

Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.

Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.