PGR Antibody

• Catalog Number: orb1273514
• Category: Antibodies
• Description: PGR Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1154
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Progesterone receptor (Ser190) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor.
• Concentration: batch dependent
• Dilution range: As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90/120
• Target: PGR
• UniProt ID: P06401
• NCBI: P06401
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020 (500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.