PGP9.5 / UchL1 Antibody

• Catalog Number: orb639550
• Category: Antibodies
• Description: This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rUBCE-L1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant UchL1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant UchL1 antibody is available for research use only.
• UniProt ID: P09936
• Storage: Store the recombinant UchL1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum with recombinant UchL1 antibody (clone rUBCE-L1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human T98G cells with recombinant UchL1 antibody cocktail (green, clone rUBCE-L1) and Nucspot (red).

Western blot testing of human brain lysate with recombinant UchL1 antibody (clone rUBCE-L1). Predicted molecular weight ~25 kDa.

Flow cytometry testing of permeabilized human T98G cells with recombinant UchL1 antibody (clone rUBCE-L1); Red=isotype control, Blue= recombinant PGP9.5 antibody.