PGLYRP1 Antibody

• Catalog Number: orb1246507
• Category: Antibodies
• Description: PGLYRP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PGNQLYHLIQN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem. 2006 Mar 3;281(9):5895-907; PMID: 16354652). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PGLYRP1
• UniProt ID: O75594
• NCBI: NP_005082.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PGRP-S, PGRPS, PGLYRP1, peptidoglycan recognition
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem. 2006 Mar 3;281(9):5895-907; PMID: 16354652). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246507 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246507 (3.8 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.